1.Oral Presentation에 있어서 실험재료와 방법(Materials & Methods)에 관한 표현
*실험방법을 선택하게 된 동기나 목적을 말 하고자 할 때,
I chose to use HPLC in order to determine the purity of each conjugates for the cell experiment.
I used MALDI-TOF mass spectrometry to confirm the peaks that appeared in the FPLC chromatogram.
*실험과정을 설명할 때
각 각의 peak가 나타내는 물질의 MW를 그 물질들의 calculated molecular weight를 이용하여 구하였다
=>I determined the molecular weight of materials represented by each peak by comparing them to their calculated molecular weight.
분자량을 알기 위해 GPC를 찍어 보았다.
-àI used GPC in order to find out the MW of …
From the GPC study, it was found that…
The results of GPC revealed that…
2주동안 1,2,4,8의 사슬을 갖는 스타형 poly(l-lactitide)를 주합하였다.
-For the past two weeks, I synthesized 4 different star-shaped PLLAs which have 1,2,4 and 8 arms, respectively.
실험에 의해 얻어진 solid를 socnication 시키면 nanoparticle을 얻을 수 있었다.
-Nanoparticles of PEG-DOCA conjugate were prepared by ultra sonication.
하지만, 30일 정도 경과 후에 같은 조건으로 sonication을 시키면 nanoparticle이 얻어지지 않았다.
-àHowever, I could not get the nanoparticles from the same sample using the same method when the PEG-DOCA conjugates were left to stand at room temperature for 30 days.
반응도중 일어나는 gelation의 변성에 의한 결과로 생각되어 반응 중 일어나는 문제점을 생각해 보려고 한다.
-This could be due to denaturation of gelatin that might have occurred during the reaction; I believe there were several problems associated with the reaction, and I would like to consider these problems with you today.
5시간동안 건조후, 건조된 젤을 60마크로 미터 두께의 여러 단편들로 자른 후, 그 단면을 SEM을 통하여 관찰하였다.
-The mixture was vacuum dried for 5 hours, after which the dried gel was cut into sections of 60mm for further observation by SEM.
30ml more than PLLA of trichloromelamine as coupling agent was introduced.
-Thirty mililiter more PLLA of thrichloromelamine was introduced as a coupling agent.
-I used 30ml more PLLA…as a coupling agent.
이것은 tricine buffer를 사용해 희석하였다.
-It was diluted by using the tricine buffer.
The enzyme used in this experiment, the concentration is the ten nanogram active mm-2.
The prepared sample was incubated in water bath at thirty seven degree C for twelve hours.
degradation시 생성되는 물질에는 어떤 것이 있고 degradation 이 된 것과 안된 것에는 hydrophillicity 에 차이가 있기 때문에 분리가 가능하다.
After the peptide is cleaved by MMPs, especially by MMP-2 and MMP-9 at tumor site, the drug has effect to kill cancer cells.
-> After the peptide is cleaved by MMPs, especially by MMP-2 and MMP-9 at tumor site, the drug is now activated and is able to kill cancer cells.